Steve Dorus


Vicens, A. and Dorus, S. The evolution of reproductive protein and the diversification of sperm. (2018) In: VIII Reproductive Journeys (Universidad Autonoma Benito Juarez de Oaxaca)
Karr, T.L. and Dorus, S. 2012. Evolutionary genomics of spermatozoa. In: Evolution in the fast lane: Rapidly evolving genes and genetic systems (eds. Singh, R., Xu J. and Kulathinal, R), Oxford University Press, Oxford, UK.
Dorus, S. and Karr, T.L. 2009. Sperm Genomics and Proteomics. In: Sperm Biology: An Evolutionary Perspective (T.R. Birkhead, D.J. Hosken and S. Pitnick, eds.), Academic Press, London
Dorus, S., Wyckoff, J. and Lahn, B.T. (2003) Mammalian Sex Chromosomes Evolution In: Nature Encyclopedia of the Human Genome (Cooper D.N.), Nature Publishing Group, London.


McDonough-Goldstein CE, Pitnick S, Dorus S. 2022. Drosophila female reproductive glands contribute to mating plug composition and the timing of sperm ejection. Proceedings of the Royal Society B 289: 20212213
McCullough E, Whittington E, Singh A, Wolfner MF, Pitnick S, Dorus S. 2022. The life history of sperm depends on molecular continuity between male and female reproductive tracts. Proceedings of the National Academy of Sciences, USA 119: e2119899119
Syed ZA, Dallai R, Nasirzadeh N, Brill JA, O’Grady PM, Cong S, Leef E, Rice S, Asif A, Nguyen S, Hansen M, Dorus S, Pitnick S. 2021. Sperm cyst “looping:” a developmental novelty that enabled extreme male ornament evolution. Cells 10: 2762
McDonough-Goldstein CE, Whittington E, McCullough EL, Buel, SM, Erdman S, Pitnick S, Dorus S. 2021. Pronounced postmating response in the Drosophila female reproductive tract fluid proteome. Molecular and Cellular Proteomics 20: 100156
McDonough-Goldstein CE, Borziak K, Pitnick S, Dorus S. 2021. Drosophila female reproductive tract expression reveals coordinated mating responses and rapidly evolving tissue-specific genes. G3 11(3): jkab020
McDonough-Goldstein CE, Pitnick S, Dorus S. 2021. Female mating status influences protein composition of mature Drosophila oocytes. Scientific Reports 11: 3142
McCullough EL, McDonough CE, Pitnick S, Dorus S. 2020. Quantitative proteomics reveals rapid divergence in the postmating response of female reproductive tracts among sibling species. Proceedings of the Royal Society B 287: 20201030
Pitnick SS, Wolfner MF, Dorus S. 2020. PEMS: post-ejaculatory modifications to sperm. Biological Reviews 95: 365-392 
Johnson SL, Borziak K, Kleffmann T, Rosengrave P, Dorus S, Gemmell N. Ovarian fluid proteome variation associates with sperm swimming speed in an externally fertilising fish. (2020) Journal of Evolutionary Biology. DOI:
Rowe M, Whittington E, Borziak K, Ravinet M, Eroukhmanoff F, Sætre GP, Dorus S. Molecular diversification of the seminal fluid proteome in a recently diverged passerine species pair. Mol Biol Evol. (2020) doi: 10.1093/molbev/msz235. PubMed PMID: 31665510. PDF
Whittington E, Karr TL, Mongue AJ, Dorus S, Walters JR. Evolutionary Proteomics Reveals Distinct Patterns of Complexity and Divergence between Lepidopteran Sperm Morphs. Genome Biol Evol. (2019) 11(7):1838-1846. doi: 10.1093/gbe/evz080. PubMed PMID: 31268533; PubMed Central PMCID: PMC6607854. PDF
Alvarez-Fernandez A, Borziak K, McDonald GC, Dorus S, Pizzari T. Female novelty and male status dynamically modulate ejaculate expenditure and seminal fluid proteome over successive matings in red junglefowl. Sci Rep. (2019) 9(1):5852. doi: 10.1038/s41598-019-41336-5. PubMed PMID: 30971704; PubMed Central PMCID: PMC6458113. PDF
Degner, E.C., Ahmed-Braimah, Y.H., Borziak, K., Wolfner, M.F., Harrington L.C., Dorus, S. (2018) Proteins, transcripts, and genetic architecture of seminal fluid and sperm in the mosquito Aedes aegypti. Molecular and Cellular Proteomics   (2019) doi: 10.1074/mcp.RA118.001067. PubMed PMID: 30552291; PubMed Central PMCID: PMC6427228. PDF
Ow, M., Borziak, K., Dorus, S. and Hall, S. (2018) Early life experiences mediate distinct gene expression and reproductive programs in Caenorhabditis elegans. PLoS Genetics 14(2): e1007219.PDF
Vicens, A., Borziak, K., Karr, T., Roldan, E. and Dorus, S. (2017) Comparative sperm proteomics in mouse species with divergent mating systems. Molecular Biology and Evolution. DOI:10.1093/molbev/msx084.
Ball, A.D., van Dijk, R.E., Dorus, S., Bowie, R.C.K., Burke, T. & Székely, T. (2016) Parental care systems reflect levels of extra-pair paternity in penduline tits (Remizidae). IBIS DOI: 10.1111/ibi.12446.
McDonough CE, Whittington E, Pitnick S, Dorus S. (2015) Proteomics of reproductive systems: Towards a molecular understanding of postmating, prezygotic reproductive barriers. Journal of Proteomics 135: 26-37. doi: 10.1016/j.jprot.2015.10.015.
Ockendon NF, O’Connell LA, Bush SJ, Monzón-Sandoval J, Barnes H, Székely T, Hofmann HA, Dorus S, Urrutia AO. (2015) Optimization of next-generation sequencing transcriptome annotation for species lacking sequenced genomes. Molecular Ecology Resources. doi: 10.1111/1755-0998.12465
Whittington, E., Zhao, Q., Borziak, K., Walters, J.R. and Dorus, S. (2015) Characterisation of the Manduca sexta Sperm Proteome: Genetic Novelty Underlying Sperm Composition in Lepidoptera. Insect Biochemistry and Molecular Biology 62:183-93.
Borziak, K, Posner, M., Upadyay, A., Danson, M., Bagby, S. and Dorus S. (2014) Comparative genomic analysis reveals 2-oxoacid dehydrogenase complex lipoylation correlation with aerobiosis in archaea. PLoS ONE 9 (1), e87063.
Freeman, Z., Dorus, S. and Waterfield, N. (2013) The KdpD/KdpE two-component system: Integrating K+ Homeostasis and Virulence” PLoS Pathogens, 9(3): e1003201. doi:10.1371/journal.ppat.1003201.
Skerget, S., Polpitiya, A., Rosenow, M., Petritis, K., Dorus, S. and Karr, T.L. (2013) The Rhesus Macaque (Macaca mulatta) Sperm Proteome. Molecular and Cellular Proteomics 12: 3052-3067
Posner, M., Upadyay, A., Crennell, S., Watson, A., Dorus, S., Danson, M. and Bagby, S. (2013) Post-translational modification in the archaea: structural characterization of multi-enzyme complex lipoylation. Biochemical Journal 449(2): 415-425.
Dorus, S., Skerget, S. and Karr, T.L. (2012) Proteomic discovery of diverse immunity molecules in mammalian spermatozoa. Systems Biology in Reproductive Medicine 58:218-228.
Fischer, B., Wasbrough, E., Meadows, L., Randlet, O., Dorus, S., Karr, T.L. and Russell, S. (2012) Drosophila paternal spermatozoal mRNA transcripts in the fertilized egg. Proceedings of The Royal Society B-Biological Sciences 279(1738) 2636-2644.
Rettie, E.C. and Dorus, S. (2012) Drosophila sperm proteome evolution: Insights from comparative genomic approaches. Spermatogenesis 2(3): 213-23.
Jackson, R.W., Vinatzer, B. Arnold, D.L, Dorus, S. and Murillo, J. (2011) The influence of the accessory genome on bacterial pathogen evolution. Mobile Genetic Elements 1(1): 55-65.
Dorus, S., Wilkin, E.C. and Karr, T.L. (2011) Expansion and functional diversification of a leucyl aminopeptidase family that encodes the major protein constituents of Drosophila sperm. BMC Genomics 12(1): 177.
Vlisidou, I.E., Eleftherianos, I., Dorus, S. Yang, G., Richard ffrench-Constant, Reynolds, S. and Waterfield, N.R. (2010) The KdpD/KdpE two-component system of Photorhabdus asymbiotica promotes bacterial survival within M. sexta hemocytes. Journal of Invertebrate Pathology, 105: 352-362.
Dorus, S., Wasbrough, E., Wilkin, E., Hester, S., Howard-Murkin, J., Lilley, K. and Karr, T.L. (2010) The Drosophila melanogaster sperm proteome-II (DmSP-II). Journal of Proteomics 73: 2171-2185. Special Issue on Model Organism Proteomics.
Dorus, S., Parker, E.R., Busby, J., and Karr, T.L. (2010) Sperm Proteomics Reveals Intensified Selection on Mouse Sperm Membrane and Acrosome Genes. Molecular Biology and Evolution 27(6):1235–1246.
Crespi, B., Summers, K. and Dorus, S. (2010) Positive selection of mental retardation genes in Rho GTPase and DNA repair pathways: insights into the evolutionary basis of human intelligence. Evolutionary Applications 3(1) 52-63.
Crespi, B., Summers, K. and Dorus, S. (2009) Genomic sister-disorders of neurodevelopment: an evolutionary approach. Evolutionary Applications 2(1): 81-100.
Dorus, S., Freeman, Z.N. and Karr, T.L. (2008) Recent origins of D. melanogaster genes encoding sperm proteins. Molecular Biology and Evolution 25(10): 2157-2166.
Waterfield, N., Sanchez-Contreras, M., Eleftherianos, I., Dowling, A., Wilkinson, P., Parkhill, J. Thomson, N., Reynolds S., Bode, H., Dorus, S., fFrench-Constant, R. (2008) Rapid Virulence Annotation (RVA): Identification of virulence factors using a bacterial genome library and multiple invertebrate hosts. Proceedings of the National Academy of Sciences 105(41): 15967-15972.
Crespi, B., Summers, K. and Dorus, S. (2007) Adaptive evolution of genes underlying schizophrenia. Proceedings of The Royal Society B-Biological Sciences 274(1627): 2801-2810.
Dorus, S., Busby, S.A., Shabanowitz, J., Hunt, D.F. and Karr, T.L. (2006) Genomic and functional evolution of the sperm proteome. Nature Genetics 38(12): 1440-1445.
Dorus, S., Anderson, J.R., Vallender, E.J., Gilbert, S.L., Zhang, L., Chemnick, L.G., Ryder, O.A., Li, W., Lahn, B.T. (2006) Sonic Hedgehog, a key development gene, experienced intensified molecular evolution in primates. Human Molecular Genetics 15(13): 2031-2037.
Loppin, B., S., Lepetit, D., Dorus, S., Couble, P. and Karr, T.L. (2005) Origin of a Drosophila paternal effect gene essential for zygote viability. Current Biology 15(2): 87-93.
Dorus, S., Vallender, E.J., Evans, P.D., Anderson, J.R., Gilbert, S.L., Mahowald, M., Wyckoff, J., Malcom, C., and Lahn, B.T. (2004) Accelerated evolution of the nervous system genes in the origin of Homo sapiens. Cell 119: 1027-1040.
Dorus, S., Evans, P.D., Choi, S., Wyckoff, J., and Lahn, B.T. (2004) Rate of molecular evolution of the seminal protein gene SEMG2 correlates with levels of female promiscuity. Nature Genetics 36: 1326-1329.
Evans, P.D., Anderson, J.R., Vallender, E.J., Gilbert, S.L., Malcolm, C.M., Dorus, S. and Lahn, B.T. (2004) Adaptive evolution of ASPM, a major determinant of cerebral cortical size in humans. Human Molecular Genetics 13(5): 489-494.
Dorus, S., Gilbert, S.L., Forster, M., Barndt, R. and Lahn, B.T. (2003) The CDY-related gene family: coordinated evolution in copy number, expression pattern, and protein sequence. Human Molecular Genetics 12(14): 1643-1650.
Dorus, S., Mimura H. and Epstein, W. (2001) Substrate-binding clusters of the K+-transporting Kdp ATPase of Escherichia coli investigated by amber suppression scanning mutagenesis. Journal of Biological Chemistry 276(13): 9590-8.
Brandon, L., Dorus, S., Epstein, W., Altendorf, K. and Jung, K. (2000) Modulation of KdpD phosphatase implicated in the physiological expression of the Kdp ATPase of Escherichia coli. Molecular Microbiology 38(5): 1086-92.